Analysis Note

ControlSK-BR-3 cells treated with vinblastine.

Application

Anti-BCL-XL (pS62) Antibody detects level of BCL-XL (pS62) & has been published & validated for use in WB.

Research CategoryApoptosis & Cancer

Research Sub CategoryBCL2 & Inhibition

For Western blotting applications, we recommend using the antibody at a 1:1000 dilution.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Bcl-xL is a ~28 kDa member of Bcl-2 family of proteins and an important regulator of apoptosis. Bcl-xL forms heterodimers with BAX, BAK, and Bcl-2, and its overexpression in tumor cells confers resistance against chemotherapeutic drugs. Bcl-xL is phosphorylated on many sites including serine 62, a critical site for Bcl-xL response to microtubule-damaging drugs such as taxol and vinblastine. Phosphorylation of serine 62 (thought to be mediated by Jun N-terminal stress kinase (JNK) signaling) negatively regulates the anti-apoptotic function of Bcl-xL and controls the growth of neoplastic cells.

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Bcl-xL that contains serine 62. The sequence is conserved in mouse and rat.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-432

Physical form

Dulbecco′s phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.3 (+/- 0.1), 50% glycerol, with 1.0 mg/mL BSA (IgG, protease free) as a carrier. 0.05% sodium azide.

Quality

Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Bcl-xL. The final product is generated by affinity chromatography using a Bcl-xL-derived peptide that is phosphorylated at serine 62.

Specificity

Human Bcl-xL. Mouse and rat Bcl-xL (100% conserved) have not been tested, but are expected to react.

Storage and Stability

Store at -20°C. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20°C. For shipment or short-term storage (up to one week), 2-8°C is sufficient.